Taqman SNP genotyping analysis

TaqMan is a commonly used SNP genotyping method developed by Life Technologies, which is an advanced, mature, validated and widely used RT-PCR technology.

Each TaqMan genotyping assay contains two primers to amplify the sequence of interest and two differently labelled allele-specific TaqMan probes for allele detection. Each allele-specific MGB probe is labelled with a fluorescent reporter dye (either a FAM or VIC reporter molecule) at the 5′ end and is coupled with a fluorescence quencher at the 3′ end.